Dna Fingerprinting

I tried to find out as much as i could this is what ive found up to now heres so information on this

DNA. It's what makes you unique. It's the stuff that tells each and every one of your body's 10 trillion cells what it's supposed to be and what it's supposed to do. And although your DNA is different from that of every other person in the world—unless you have an identical twin—it's the same in every cell that makes up your body. That DNA is unique from person to person but the same from cell to cell in one person can be a good thing, especially when it comes to DNA fingerprinting. DNA fingerprints can be used for anything from determining a biological mother or father to identifying the suspect of a crime. And it can be used to clear someone's name. But what exactly is a DNA fingerprint? Well, it certainly isn't an inky impression of a DNA strand. Compared to microscopic small DNA, a fingerprint is HUGE. So what is it that we're looking at?, and how is one of these fingerprints made?

This next information is in a dilema then how to solve it I will then write as many facts as i can down afterwards to make it just a bit more scientific:

The Crime

On the evening of November 1, at approximately 8:15 p.m., Jimmy Sweet entered his bedroom, walked over to his desk, and sat down at his computer. While reaching for the computer's switch he noticed, out of the corner of his eye, that one of the items on his well-organized shelf was out of place. Jimmy shot across the room for a closer look. Sure enough, the object which was out of place had indeed been disturbed. The object had been sealed in an air-tight package. The package was now ripped open. The object was still inside, but it was no longer in its original condition. In Jimmy's eyes, it was now worthless. Jimmy pulled out what had been his most-valued possession—his holographic NOVA lollipop. The confectionery treat was now a sticky mess. Someone had obviously indulged him- or herself in its sugary goodness . The lollipop's holographic image had been licked away.

The Suspects

The prime suspects in this case are Jimmy's seven sisters: Natalie,Amy,Lucy,Ashley,Jane,Jodie and Beth. Each one of these sisters is a extremely big candy lover and is easily capable of committing the crime. The suspects have been held. DNA fingerprints of each are available.

What we need to do

Fortunately for this case, a lollipop cannot be licked without leaving behind a bit of saliva. Your task, as NOVA Lab's chief technician, is to create a DNA fingerprint from the saliva left on the lollipop. You must then use the fingerprint to single out the culprit of this crime.

At the DNA fingerprinting lab

The DNA specimen on the lab countertop has already been prepared, using some of the saliva extracted from the lollipop

Instructions on how to do this are as follows:

1Pour restriction enzymes into dna-Restriction enzymes work like scissors cutting along the dna molecules at diffrent places. Depending on where they cut depends on the code in the dna and in the enzymes.The lengths of these pieces depends on the codes and the codes are all different.Some will be longer and some shorter.

2Pour agrose gel into a tray on the lab counter-Agrose is a thick gel-like substance.It acts a a molecule strainer allowing smaler pieces of dna through quicker than the larger ones

3Make a hole in the Agrose gel and then pour the DNA into the tray -The dna now lies in this hole

4Turn on a switch to begin electrophoresis-The process of moving molecuses using an electrical current.The dna fragments have a small negative charge so they move towards the trays positive end (Like magnets opposites attract) But with the gel being like a strainer the smaller fragments move more easier and get further along the tray to the positive end more than the longer ones.When the process is finished the fragments are distributed Biggest (at mainly negative side) to smallest (at mainly positive side)

5Place a nylon membrane on top of the gel-Because agrose gel is particulary hard to work with a nylon membrane(a bit like paper) is placed over the top which attracts the dna (a bit like sucking it up) and makes the dna print to that instead.

6Add probes onto the nylon gel-Probes are pieces of dna that have been radioactively labeled.The probes attach themselves to the dna on the nylon membrane

7Place X-ray film on top of the nylon membrane-The radioactivity from the probes,which are only present at a few places on the nylon membrane,exposing to corresponding areas on the x-ray film

8Develop a film by putting it in a developer-The x-ray film has been developed.The film shows the places on the nylon membrane were the probes attached themselves to the DNA fragments.This is a DNA finger print

Blood

Well I have surfed the net and found everything about blood

Blood is a specialized bodily fluid that delivers necessary substances to the body's cells—such as nutrients and oxygen—and transports waste products away from those same cells. Causing blood to be necessary for human life.

Blood is plasma and is usually yellow but red blood cells make blood red.

Blood cells are designed for this purpose as they don't have a nucleus so can carry more blood. Blood cells are produced in the bone marrow

We all have these little markers called antigens on the surface of our red blood cells. These are so tiny they can't even be seen under a microscope.Every one's has different types - blame your parents and grandparents for all that.In fact, only identical twins will ever possess all the same antigens. There may be complications when having blood transfusions as some peoples blood is different than others.
There are 4 main blood groups A, B, AB and O, of which group O is the most common (47% of population). The blood type is determined by proteins called antigens found on the surface of red blood cells. If you have the antigen A on the red blood cells then you have got type A blood. When B antigen is present, you have type B blood, when both A and B are present, you have type AB blood. When neither are present you have type O blood.

Another blood group system involves Rhesus factors. The name Rhesus comes from the Rhesus monkeys in which the protein was first discovered. Rhesus factor D, the most important, is found in the blood of 85% of people, they are known as Rhesus positive. The remaining 15% are Rhesus negative. So people can be classified according to both systems, for example AB positive or O negative.
The Rhesus factor is important during pregnancy, a baby's life can be endangered if it inherits a Rhesus positive blood type from its father while the mother is Rhesus negative. This is because the mother can form antibodies against the baby's blood causing the mother to think the baby as a germ or infection

Blood is a very complex substance of the human body that often helps crime investigators to find the offender or any other clues connected with the crime. Let us investigate in more detail what blood consists of, how it is identified and distinguished. One of the major qualities of blood used by criminal investigators is the ability to clot.

Clotting is a helpful key to crime scene investigations as it can from an estimate guide to how much time has passed since the blood has left the body. Blood will start to clot between 3-15 minutes after it has left the body, but this can be affected by haemophilia, leukaemia and various medications. When the blood starts to clot it turns into a dark, shiny, jelly-like mass and after sometime it will start to contract and separate from the serum.
If the blood is found in liquid form then the bleeding only happened a few minutes before, if it is a shiny, gelatinous pool, then bleeding occurred less than an hour ago and if the blood is separated into a clot and serum than several hours have passed.

Blood is the most common bodily fluid at crime scenes and is the most useful due to the way it moves and clots. Blood stains left at an accident, suicide or crime scene can help determine what happened or if a crime was even committed. Other than revealing blood types, blood can also reveal the presence of diseases, drugs or alcohol and is used to determine the identification of the victim and suspect through DNA analysis. The shape and location of the blood stains also provide clues about where the victim and suspect where when the crime took place and where they went afterwards.
Blood spatter
A blood spatter is a group of blood stains resulting from one or more injury. Spatters are produced in several different ways such as stabbing, gunshots, beatings, arterial bleeding, cast-off blood, and splashing.
Bloodstains are categorised as either passive or projected patterns and a careful analysis of these patterns can provide the following information:
• The origin of the bloodstains • The type of instrument that caused the bloodstains (edged, blunt, firearm, etc.) • The direction from which an object struck the victim (by calculating angles of impact) • The relative positions of the victim and suspect • The locations and movements of the victim and suspect during the attack • The number of blows or gunshots the victim received • The truthfulness of any suspects and witnesses.
Each droplet of blood strikes a surface in a unique angle and direction. The impact angle is the slant at which the blood drops strike the surface, and the directionality is the course the blood drop followed. The impact angle is found out by using a protractor and the directionality of each stain helps determine the point of convergence and point of origin. This is normally done by strings being stretched according to and alongside the angle of impact.

Passive blood spatter

A passive stain is created by the force of gravity alone and can create three different types of patterns: • Passive Drop - Bloodstain drops created or formed by the force of gravity acting alone. • Drip Pattern - A bloodstain pattern which results from blood dripping from an injured persons wounds, a blood-covered weapon or object, the assaliants hands, or any elevated object. • Flow Pattern - A change in the shape and direction of a bloodstain due to the influence of gravity or movement of the object.
A drop of blood is formed due to a smaller amount breaking away from a larger blood source and due to surface tension it remains spherical until they strick or are struck by an object or surface. When a drops hits a surface it creates a circular pattern around the point of impact. The shape and size of the circular patter all depends of the size of the drop, the speed in which it fell, the angle it hits the surface and the type of surface it strikes.
A blood drop will pick up speed as it falls, and this, with the distance the drop falls from an inch to 7 feet, will produce the sahpe and size of the circular patterns diameter. The diameter of the spatter pattern can vary from 13mm to 22 mm.
When a drop strikes a surface from 90 degrees the spatter will form an even circle at the point if impact. If the blood falls from an angle smaller than this, it creates a longer oval pattern with a narrow end aiming in the drops direction of travel.
Blood will behave differently on certain types of surfaces. Hard smooth surfaces such as glass with produce a smaller and neater spatter than rough irregular surfaces like concrete.

Projected blood spatters

Projected blood spatters occur when a force other than gravity is applied. The size, shape, and number of resulting stains will depend on the amount of force applied to the strike.
Projected blood is classified in one of two ways, by velocity;• Low Velocity - A bloodstain pattern that is caused by a low velocity impact or force to a blood source. This impact results in a fairly large spatter. This includes arterial bleeding, which is blood loss in the form of spurts or gushes when an artery is damaged. Arterial bleeding patterns normally result in a cascading spatter. Cast-off blood is another low velocity blood source. This is blood that is ‘flung’ from an object in a centrifugal force, this usually happens when a seris of arching blows is delivered and the patterns are usually found on walls and ceilings. These spatter patterns can help with estimating the height and whether the assailant is left or right handed it can also indicate the minimum number of blows to the victim. • Medium Velocity –A bloodstain pattern caused by a medium velocity impact or force to a blood source. These type of spatters are normally smaller than those from low-velocity droplets and tend to come from impacts with blunt or sharp objects which distribuate blood in all directions from the source of impact. Again these can help determine the point of origin. Sometimes a fine mist spatter is produced and this is caused by any wounds made to the troat, face or lungs as the blood mixes with exhaled air. The fine spray may be found on or around the victim and the attacker. • High Velocity - A bloodstain pattern caused by a high velocity impact or force to a blood source. The spatter produced is a mist like stain. These are normally associtaed with gun shot wounds as bullets travel at a high velocity. These patterns tend to produce an exit or entrence wound. A blood spatter showing an entrance woud is called blowback or back spatter and this is when the blood travels in the opposite direction to the path of the object. A bloodstain found near the exit wound is called forward spatter in which the blood follows the path of the object. Projected blood spatters are also classified by type of spatter:• Impact spatter- this occurs when a foreign object has impact with the victim. These include stabbing, gunshots and beatings. • Projection spatter- these are the results from arterial bleeding, cast-off blood and exhaled blood. • Combination spatters- include a mixture of impact and projection spatters. These are the types of spatters that are frequently found at crime scenes.

Transfer patterns
Transfer patters are the results of when blood soaked objects come into contact with another object. These can include bloody shoe prints, hand prints and finger prints. A transfer patter normally happens when someone brushes against or kneels in a bloodstain, or wipes and cleans their hands or weapons resulting in the victims blood being transferred on the suspects clothing or onto floors and walls. Matching the victim’s blood to a transfer stain found on the suspects clothing may help in solving the crime!

FingerPrints

Well after looking at all the information I found youll be glad to not have to read it yourself but heres the most important parts-

Finger prints are raised ridges in the finger that create patterns

No two fingerprints are the same even identical twins have different finger prints!

There is four main types of finger prints:

arch

whorl

loop

tented arch








Crime scene investigators(thats me) have to take picture,scan the area with a special machine(kevin probe detects the fingerprint)and use a special powder to make the ridges stand out more.Fingerprints can be left on about anything such as motor oil,blood,paint,ink ect.


There are three types of print latent-any chance or accidental impression left by raised parts of a fingerprint,this way isnt very good as the print may become distorted and or smuged making it difficult to see the fingerprint which may lead to wrongly accusing someone. anothe way is patent-these are prints which are easy to see with your eyes and are caused by another material getting onto the finger and then printing it somewhere else an example of this an inkpad,these prints are usually to take a picture.Plastic prints are a print which has been put nto somthing which has ket the shape of the ridges of the fingers,examples which are commonly saw are fingers put in window putty,melted candle wax and thick grease deposits on car parts.You then take photos of the mould then try and make non-plastic copies

Electrical detection by rolling the finger on a scanning machine which will detect the raised ridges in the finger.But when there is too much pressure on the finger it can cause distortions in the end product

Another is using powder to discover the ridges in the skin by making the ridges stand out on the surface it was put on.

The kevin probe is scanned over the ared of the fingerprint it detects voltage, or electrical potential, at pre-set intervals over the surface of an object on which a fingerprint may have been put! The results produce a map or image of the fingerprint, the probe is used mainly on fingerprints which have been put on metals as they are easier to detect.

Well I have done a lot here I hope the constable will be happy with my progress=]

Introduction To CSI

Hello,Ive just started a job as a crime scene investigator and I have been asked to find out information on forensic science. I was then asked to make it into a blog so people who are also new to the job can also just look at this instead of finding information themselves then can just read mine. What a clever idea!!
So in my job I need to learn about :
Fingerprints
Bloodtypes
DNA Fingerprinting
Chromatography
Microsopes
and then finally my case study
Hope you learn lots because I know I will!
Please leave comments on how I am doing =)